Abstract: Spatial-temporal coupling characteristics of interaction between urbanization and eco-environment in Hunan since 2001 are analyzed empirically based on the framework of “analysis of system evolution characteristics-validation of dynamic coupling relationship-coordination status diagnosis and early warning”. The results show that: (1) the comprehensive development index of urbanization in Hunan Province increased from 0.537 in 2001 to 0.785 in 2015, reaching a middle and high level, and Changsha is the only city which achieves high level in 14 cities; (2) the index of ecosystem health showed a trend of U, reaching 0.764 in 2015 and in sub-health state, and greater system pressure was the key limiting factor; (3) the degree of coordination between urbanization and eco-environment first declined and then increased to 0.622 in 2015, which was at a lower level of basic coordination, and most of the cities and towns were in the critical value of mild disorders to basic coordination, which belonged to three types: lagging behind ecological construction, lagging urbanization and structural pollution; (4) urbanization and eco-environment have changed from mild disorders-regressive succession to mild disorders-positive succession and further to basic coordination-positive succession from the overall perspective of system evolution, the coupling degree of urbanization and eco-environment will be increased to 0.633 in 2020; Hunan will be in the green area for a long time in the future, and appropriate regional development policies should be adopted to promote the healthy development of urbanization and eco-environment.