Abstract: The spatial distribution characteristics of habitat quality in Huangshan city, a typical tourist city, and their influencing factors mechanisms were analyzed in this paper by using remote sensing image classification and interpretation data, meteorological data, statistical yearbook data, Baidu map data and other multi-source data in 2017, comprehensive models of ecosystem service value, Habitat quality index consisting of NPP and NDVI, InVEST habitat quality assessment model based on external threats and geo-detector. The results show that: (1) The total value of habitat quality in Huangshan City in 2017 was 1.84×1010 yuan , and the average value of habitat quality was 14694 yuan/hm2. The habitat quality in the west and north of Huangshan city was overall higher than that in the east and south. (2) The habitat quality of Qimen County was the best in Huangshan city, while the Tunxi District was the worst. The average value of habitat quality in Longmen Township adjacent to Taiping Lake was the highest in all towns in Huangshan, and the lowest was Yuzhong Street. (3) Topography was an important basic factor affecting the spatial distribution of habitat quality. The interpretation of altitude, terrain fluctuation, slope and slope difference on spatial differentiation of habitat quality were all above 0.6, and the slope had more influence on habitat quality than altitude. (4) Tourism activity was an important external driving force for the spatial differentiation of habitat quality in huangshan city, and the correlation between habitat quality and potential determinants was higher in tourist areas than in other areas. The area with High-level tourism resources gather, convenient traffic, and flat terrain, as a large tourist reception and distribution center, became a low-value area of habitat quality. The area with high-quality tourism resources gather, but limited by traffic, location, terrain and resource protection, became a medium quality habitat area. While the area where tourism resources were scattered, and the transportation was inconvenient, the quality of habitat was less affected by human, became a high quality habitat area. And space theory can be used to explain the spatial differentiation mechanism of habitat quality．