Abstract: Equalization of rural medical and health services is the main goal of the national rural revitalization plan. Driven by the integration of urban and rural transportation, motorized travel has become the priority preference of the majority of rural households for transportation travel. Under this background, the research on the equalization of rural medical and health services is of urgency and necessity. This paper takes Jiangsu as an example, based on the GIS network analysis method to measure the accessibility of rural medical and health services under walking, electric bicycle and automobiles travel modes respectively, and tries to reveal the rural medical and health services accessibility and the matching characteristics of supply and demand driven by the integration of urban and rural transportation. This study have shown that the rural medical and health accessibility is low in walking mode, while electric bicycles can ensure that most rural areas enjoy higher level access to health service stations, and automobiles enable most farmers to enjoy higher level hospital accessibility. Integration of urban and rural transportation is a key factor to promote the equalization of rural medical and health services, which can effectively solve the problems of insufficient rural medical facilities and health services, inconsistent supply and demand, and low utilization during the process of equalization of rural medical services. In addition integration of urban and rural transportation can create the necessary conditions for sharing high-quality medical and health services for farmers. In view of this, in order to achieve the goal of equalization of urban and rural medical and health services, we should continue to accelerate the pace of urban and rural transportation integration, discuss the project design of medical and health service system planning under the framework of urban and rural transportation integration, and optimize the layout of urban and rural medical and health services.