Abstract: Abstract：At present, there were few researches on the influence of human activity intensity on wetland landscape pattern at large scale. To reveal the relationship between the wetland landscape pattern in the middle and lower reaches of the Yangtze River Basin and human activity intensity, the paper took the land use data of the middle and lower reaches of the Yangtze River Basin from 1995 to 2015 as data sources. Human activity intensity of land surface (HAILS), and landscape index were comprehensively used to analyze the evolution characteristics of human activity intensity, wetland landscape pattern characteristics and the influence of human activity intensity on wetland landscape pattern. The results showed that: (1) The area of high human activity intensity zone increased, while the area of low human activity intensity zone decreased; the average value of each human activity intensity zone showed an increasing trend from 1995 to 2015, and the higher of the intensity zone grade, the greater of the increasing range of average value. (2) In the past 20 years, the wetland area has been increasing, accounting for 5.14%, 5.27% and 5.30% respectively; lakes and marshes have decreased by 70.73 km² and 216.53 km² respectively, while the area of reservoir pond has increased by 1 155.32 km2, contributing 94.75% to the increase of wetland. It indicated that artificial wetlands were expanding while natural wetlands were shrinking during the study period. The fragmentation of wetland landscape was weakened, and the landscape diversity was decreased. (3) The relative change of wetlands showed a ‘contraction’ effect with the increase of human activity intensity, and the higher human activity intensity, the more obvious of the ‘contraction’ effect. With the decrease of human activity intensity, the fragmentation of wetland landscape was weakened, the aggregation reduced, the landscape diversity enhanced and the landscape shape tended to be complex. The research results can provide decision support for wetland protection and human activity control in the middle and lower reaches of the Yangtze River Basin.