Abstract: In this paper, taking 11 provinces and cities in the Yangtze River Economic Belt as the research object, the panel data from 2009 to 2018 is selected, and the green innovation efficiency of high-tech industries in various provinces and cities is calculated by Using Matlab Software based on the Super-SBM model considering undesirable output, constructing a comprehensive index of Internet development through principal component analysis. Finally, the two-way fixed effect model is used as a benchmark model to explore the role of Internet development and environmental regulation on the efficiency of green innovation in high-tech industries. Studies show that: (1) Under the condition of only considering regional effects and not adding environmental regulatory variables, the development of the Internet plays a positive role in the green innovation efficiency of high-tech industries.  (2) When considering regional effects and time effects, environmental regulations inhibit the improvement of green innovation efficiency of high-tech industries, while the development of the Internet also plays a role in inhibition, and the synergy between Internet development and environmental regulation can promote the improvement of green innovation efficiency of high-tech industries.  (3) Finally, it proposes to make full use of Internet technology, adopt differentiated environmental regulatory policies and integrate the development of the Internet and environmental regulations, with a view to improving the green innovation efficiency of high-tech industries in the Yangtze River Economic Belt.