Abstract: The change of urban green space pattern leads to the change of ecosystem services during ecological transformation of mining cities. In this paper, Xuzhou City, a typical mining city, was selected as the research site. Green space information in 2000, 2010 and 2020 was extracted via remote sensing images. Landscape pattern index method was used to explore the evolution of green space pattern to evaluate four ecosystem services, including food supply, carbon storage, soil conservation and habitat quality in order to explore the tradeoff and cooperative relationship of ecosystem services in the process of ecological transformation. The results indicated: (1) The green space in Xuzhou accounted for more than 70% of the total area of the research site in 2000-2020, but with a gradual decline. On the one hand, a large amount of green spaces was transformed into construction land along with the rapid urbanization process; on the other hand, part of the non-green spaces was transformed into green spaces due to urban ecological construction and ecological restoration of abandoned mining areas, including the central urban area, built-up area in Jiawang District and coal mining subsidence area. (2) The heterogeneity of urban green space landscape in Xuzhou has been declining, which somewhat relieves the degree of fragmentation. The landscape form has been becoming intact gradually with a reasonable connectivity. (3) Influenced by the change of green space pattern, food supply quantity of green space continues to increase, and soil conservation decreases first and then increases, and the carbon storage and habitat quality declines continuously. The ecosystem service level presents a spatial characteristics of “high in the periphery and low in the middle”. (4) In Xuzhou’s ecological system, tradeoff relationship exists between supply services (food supply) and regulation services (carbon storage and soil conservation), and between supply services and support services (habitat quality). Mutual gain-based cooperative effect exists between regulation services (carbon storage and soil conservation) and support services (habitat quality).