Abstract: Investing in natural capital (NC) and improving the ecosystem services value (ESV) are important measures to promote economic growth and environmental protection cooperatively. One of the keys is to measure ecosystem services value. Based on the survey data of various land-use types in Chongqing from 2009 to 2016, The spatiotemporal distribution pattern of ESV in Chongqing is measured and calculated, then the spatio-temporal dynamic relationships between ESV and economic growth are identified during the study period. The results indicate that: (1) the ESV of Chongqing was respectively RMB 401.129, 400.063 and 403.724 billion in 2009, 2012, and 2016, respectively, documenting a U-shaped curve of decrease first and then increase, and ESV in the northeastern and southeastern parts of Chongqing was obviously higher than that of main city zones and western Chongqing. (2) The main reason for the trend is the change of forest land area. The occupancy of construction land led to the decline in the early stage, while the implementation of ecological projects such as conversion of farmland to forests, protection of natural forest resources and subsequent vegetation restoration of the Three Gorges Project promoted the increase of ESV in the later stage. (3) The negative correlations between GDP per capita and AESV of Chongqing were weakened according to spatial autocorrelation, which is manifested as “integrated high and low values took the main part, with dual high values and dual low values as auxiliary parts”, of which low-high clustering regions were distributed in northeastern and southeastern parts and high-high clustering regions were distributed in western parts and main city zone of Chongqing. Finally, decision-making references and suggestions would be put forward in this thesis to realize the dual growths on both economy and ecology in various districts and counties according to their local conditions in Chongqing.