Abstract: In order to understand and protect the environment and resources of the Dajiuhu wetland park, 12 field surveys of vertebrates were conducted between 2016 and 2018. A total of 232 species (26 orders and 78 families) of vertebrates were recorded, including 12 fishes species (2 orders and 2 families), 12 amphibians species (2 orders and 5 families), 14 reptiles species (1 order and 6 families), 167 birds species (16 orders and 52 families), and 27 mammals species (5 orders and 13 families). Fauna analysis showed that there were 114 oriental realm species, 69 palaearctic realm species, and 37 widespread species. Seventy-nine species were listed as national or provincial key protected animals, represented by Aquila chrysaetos, Haliaeetus albicilla and Ursus thibetanus. The analysis of Shannon-Wiener diversity index and Pielou evenness shows that diversity was represented by birds > mammals > reptiles > amphibians > fishes; evenness is represented by reptiles>birds>mammals >amphibians>fishes. The results show that vertebrates in the Dajiuhu wetland park have the characteristics of abundant species, higher diversity, more national-level protected animals, lower population density, less dominant species, abundant wetland birds, and more oriental realm species, playing an important biological group of wetland and shrub ecosystems.