Abstract: In order to reveal the diurnal variation of surface soil respiration and its influencing factors in the dolomite region of Shuanghedong National Geopark, Suiyang, Guizhou. In this study, the soil respiration measurement system (SRS-SD2000, ADC, UK) was used to monitor the four different land use methods of shrub forest, bamboo forest, dry land and artificial grassland in the karst area of Shuanghedong National Geopark without the influence of precipitation The characteristics of the diurnal variation of the surface soil respiration flux, combined with environmental parameters such as soil temperature and humidity and atmospheric temperature and humidity, explore the key factors affecting the diurnal variation of soil respiration. The results showed that the soil respiration rates of the four different land use patterns all showed a unimodal pattern of “the day is high and the night is low”, with the maximum daily variation occurring 12∶00-14∶00 and the lowest occurring at 5∶00-7∶00; the soil respiration rate and soil temperature in each plot showed a significant positive correlation (P<0.001), and the soil respiration rate in shrubbery land, bamboo forest, and dry land had a significant negative correlation with soil moisture (P<0.001), there is a low correlation between soil respiration and soil moisture in artificial grassland (P<0.05); air temperature is the key factor affecting the change of soil respiration rate. The measured data show that soil respiration will not continue with the temperature after reaching the peak There are differences in the day and night response characteristics of soil respiration rate to soil temperature and humidity.