Abstract: In order to provide reference for the improvement of regional ecological environment and rational utilization of land, this paper analyzed the characteristics and spatial evolution of land ecosystem service value during the four periods from 1990 to 2016 in Wuhan. Based on Landsat TM image interpretation data, this paper used adjusted equivalent factor valuation model of ecosystem service to compute ecosystem services value and further utilized spatial analysis tools to explore spatial features of the value changes. The results showed that: (1) The ecosystem services value in Wuhan showed a trend of first decreasing and then increasing. The ecosystem services value of rivers and lakes was always the highest in all periods, followed by that of farmland, pond and wetland. These three types of ecosystems provided Wuhan More than 85% of ecosystem service value. (2) The spatial distribution of the total value of ecosystem services in each township was characterized by spatial agglomeration and the degree of correlation tended to increase. (3) The spatial correlation of the ecosystem service value change rate in each township was weakened continuously. In the later stage, discrete effect appeared. (4) The unbalanced spatial distribution of ecosystem services in central urban areas tended to spread to the suburbs. The location conditions of townships as well as the proportion of rivers and lakes were closely related to the changes of the total value of ecosystem services in urban areas. The research showed that the spatial distribution of urban ecosystem services value was greatly influenced by the urban expansion directions and the change of land use structure. Rivers and lakes played an important role in improving urban ecological environment.