Abstract: Based on the remote sensing image interpretation data of land use/cover change (LUCC) in Jiangsu Province in 2005, 2010, 2015, and 2018, the equivalent factor in the second-level classification ecosystem was applied to quantitatively assess the total value of ecosystem services and the function value of single ecological service in Jiangsu Province. Then, the spatial autocorrelation analysis method was adopted to study the temporal-spatial evolution characteristics of ecological service value changes in each county unit. The results showed that: (1) From 2005 to 2018, the area of cultivated land, forest land and grassland in Jiangsu Province decreased, while the area of wetland, construction land and unused land increased. The increase and decrease of cultivated land, wetland, and construction land area in each period dominated the regional land use change. (2) From 2005 to 2018, the total value of ecosystem services in Jiangsu Province increased by 60.076 2×108 yuan. Hydrological regulation was the leading ecological service function. Maintaining wetland area not only led the change of the total value of ecosystem services, but also was the key to maintain the value of hydrological regulation services and ensure regional ecological security. (3) The regional average value of ESV in all counties over the years showed significant positive spatial correlation, with local spatial agglomeration. The temporal-spatial evolution in the high-value cluster area remained stable. The massive occupation of cultivated land and forest land by construction land during the rapid urbanization development and the regional effective wetland protection and restoration work were the important reasons for the evolution of low-value cluster area and spatial differentiation characteristics of the regional average ESV variation. The research results can provide scientific basis to formulate scientific and reasonable ecological protection policies and establish long-term mechanism of ecological protection and restoration for Jiangsu Province.