Abstract: As the urbanization processing rapidly, the spatial pattern of village and town settlement is transforming and reshaping. And it is of great significance to probe into the change of village and town settlement and the mechanism behind the phenomenon. In the work, based on the multi-temporal remote sensing images in 2000 and 2015, the spatial pattern characteristics of village and town settlement in Jiangsu province were analyzed by using the methods of nearest neighbor ratio, kernel density and spatial auto-correlation, and on the basis of the provincial and county scale the spatial change of settlement was discussed, and then the driving mechanism of driving force mechanism was constructed. Conclusions are drawn as follows: (1) On provincial scale, the distribution of village and town settlement is concentrate, but there is a weakening trend of the concentration. The regional difference is significant, since the western part of Jiangsu Province has higher density than the east, and in north the high density agglomeration is continuous while the south is fragmented. Overall, the density cores, especially the density cores in Southern Jiangsu, are shrinking obviously. (2) On county scale, the distribution of village and town settlement is concentrate still. However, the concentration is weakening from 2000 to 2015, which is consistent to the provincial aspect. The distribution of cold and hot spots is relatively scattered. The hot spots mainly distribute in Northern Jiangsu, while the cold spots locate in Southern Jiangsu only. Over time, the hot spots is expand and the cold spots is shrinking. So from the county aspect, the change of the hot and cold spots is both obvious in Southern and Northern Jiangsu. (3) In terms of evolution mechanism, natural geography, social economy, policy and other factors are all considered. However, from the degree of the change that factors made, transportation, urbanization and policy play the most important roles. These three factors account for the spatial change of village and town settlement in Jiangsu province from 2000 to 2015.