西昌高原鳅人工繁殖技术研究

doi:10.11870/cjlyzyyhj2016Z1006

长江流域资源与环境 >> 2016, Vol. 25 >> Issue (Z1): 38-43.doi: 10.11870/cjlyzyyhj2016Z1006

西昌高原鳅人工繁殖技术研究

李荣¹, 吴湘香¹, 吴世勇², 李云峰¹, 王红梅², 茹辉军¹, 盛强¹, 张燕¹, 甘维熊², 陈修松², 倪朝辉¹

1. 农业部长江中上游渔业生态环境监测中心, 中国水产科学研究院长江水产研究所, 湖北武汉 430223;
2. 西昌学院邛海湿地与环境保护重点实验室, 四川西昌 615013

收稿日期:2016-06-06 修回日期:2016-06-14 出版日期:2016-11-26
通讯作者: 倪朝辉,E-mail:nizhaoh@yfi.ac.cn E-mail:nizhaoh@yfi.ac.cn
作者简介:李荣(1962~),男,硕士,主要研究方向为长江中上游渔业生态环境监测与保护.E-mail:lrqm@yfi.ac.cn
基金资助:
雅砻江流域水电开发有限公司项目（JPIA-G201224）

STUDIES ON ARTIFICIAL PROPAGATION TECHNIQUES OF TRIPLOPHYSA XICHANGENSIS

LI Rong¹, WU Xiang-xiang¹, WU Shi-yong², LI Yun-feng¹, WANG Hong-mei², RU Hui-jun¹, SHENG Qiang¹, ZHANG Yan¹, GAN Wei-xiong², CHEN Xiu-song², NI Zhao-hui¹

1. Fishery Eco-environmental Monitoring Center in the Upper and Middle Reaches of the Yangtze River, Ministry of Agriculture/Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China;
2. Key Laboratory of Qionghai Wetland Environmental Protection in Xichang College, Xichang 615013, China

Received:2016-06-06 Revised:2016-06-14 Online:2016-11-26
Supported by:
Project of Yalong River Hydropower Developmental Company(JPIA-G201224)

摘要/Abstract

摘要： 对西昌高原鳅（Triplophysa xichangensis）人工繁殖中的催产药物及其剂量、受精方式、孵化方法和仔鱼水花培育方法等一系列关键技术进行了详细研究。结果表明，对性腺发育至Ⅳ⁺~Ⅳ⁺⁺期的雌鱼分二次注射LRH-A₂ 0.1 μg/g（鱼体重）和HCG 20 IU/g（鱼体重），雄鱼剂量减半，注射量0.1 mL，能很好地促进雌雄鱼发情并产卵排精，在水温19±0.5°时的效应时间为30.5~31 h；在一个特制的塑料盆中完成了亲鱼培育、打针催产、发情、自然产卵受精、孵化以及仔鱼培育等全部人工繁殖过程，受精率、孵化率和仔鱼水花培育20 d的成活率平均分别为97.2%、85%和85%。本研究成果可应用于西昌高原鳅规模化繁育，对长江上游鱼类生物多样性保护有着积极意义。

关键词: 西昌高原鳅, 人工繁殖, 催产, 受精, 开口饵料

Abstract: In this study we carried out a series of experiments on every key technologies of the artificial propagation of Triplophysa xichangensis, including oxytocin drugs and dosage, the insemination method, and the larval hatch and culture method. The results showed that, when the gonad of the female fish developed to the Ⅳ+-Ⅳ++ period, twice injection was given with 0.1 ml hormone containing LRH-A at a dose of 0.1 μg/g fish and HCG at a dose of 20 IU/g fish, while the doses were halved for male fish, it could preferably promote brood stock estrus and spawn. The response and effective time of the hormone was 30.5-31h at a water temperature of 19±0.5℃. All the artificial propagation processes of the fish, including the intensive cultivation of brood stock, hormone injection and natural spawning, hatching of fertilized eggs, cultivation of larvae fish, were carried out in a special plastic basin. The fertilization rate and hatching rate was 97.2% and 85%, respectively, and the survival rate of the 20d larvae fish was 85%. This research can be applied in large-scale breeding of Triplophysa xichangensis, and has positive significance for the protection of fish biodiversity in the upper Yangtze River.

Key words: triplophysa xichangensis, artificial propagation, oxytocin, fertilization, first feed

中图分类号:

李荣, 吴湘香, 吴世勇, 李云峰, 王红梅, 茹辉军, 盛强, 张燕, 甘维熊, 陈修松, 倪朝辉. 西昌高原鳅人工繁殖技术研究[J]. 长江流域资源与环境, 2016, 25(Z1): 38-43.

LI Rong, WU Xiang-xiang, WU Shi-yong, LI Yun-feng, WANG Hong-mei, RU Hui-jun, SHENG Qiang, ZHANG Yan, GAN Wei-xiong, CHEN Xiu-song, NI Zhao-hui. STUDIES ON ARTIFICIAL PROPAGATION TECHNIQUES OF TRIPLOPHYSA XICHANGENSIS[J]. RESOURCES AND ENVIRONMENT IN THE YANGTZE BASIN, 2016, 25(Z1): 38-43.

参考文献

[1] 肖海, 代应贵. 中国高原鳅属多样性研究概述[J].水产科学, 2011, 30(1):53-57.[XIAO H,DAI Y G. A Review of Study on Diversity of Triplophysa in China[J]. FISHERIES SCIENCE,2011, 30(1):53-57.]
[2] 四川省人民政府.四川省新增重点保护野生动物名录(2008,08,15)[EB/OL]. http://www.docin.com/p-254864018.html.
[3] ENDO T,ISHIDA M,YAZAWA R, et al. Mass production of fertilized eggs by artificial insemination from captive-reared Pacific bluefin tuna(Thunnus orientalis)[J]. Aquaculture,2016, 451:72-77.
[4] 陈修松,陈娇,邓思红,等. 西昌高原鳅人工繁殖及苗种培育研究[J].现代农业科技,2015,3,287-289.[CHEN X S,CHEN J,DENG S H,et al. Research on Artifical Breeding and Fry Cultivation of Triplophysa xichangensis[J].Modern Agricultural Science and Technology.2013,287-289.]
[5] 刘鉴毅, 危起伟, 杜浩, 等.中华鲟人工繁殖关键技术的改进效果研究[J]. 经济动物学报, 2006, 10(2):96-100.[LIU J Y, WEI Q W, DU H, et al. Studies on Promoted Key Technologies in Artificial Propagation of Chinese Sturgeon[J]. Journal of Economic Animal, 2006, 10(2):96-100.]
[6] 王武. 鱼类增养殖学[M]. 北京:中国农业出版社, 2010, 181-215.
[7] 刘鹏斌, 缪丽梅, 高杰, 等. 大鳍鼓鳔鳅人工繁殖技术初探[J]. 内蒙古农业大学学报, 2013, 34(4):1-3.[LIU P B,MIAO L M,GAO Jie. et al. STUDY ON ARTIFICIAL BREEDING TECHNIQUE OF Herzenstein[J]. Journal of Inner Mongolia Agricultural University, 2013, 34(4):1-3]
[8] 梁银铨, 胡小建. 长薄鳅人工繁殖技术的研究[J]. 水生生物学报, 2001, 25(4):422-424.[LIANG Y Q,HU X J. STUDIES ON ARTIFICIAL PROPAGATION OF LEPTOBOTIA ELONGATA[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(4):422-424.]
[9] 李强, 姚明予, 周波,等. 中华沙鳅人工繁殖技术初探[J]. 淡水渔业, 2011, 41(5):92-95.[LI Q,YAO M Y,ZHOU B, et al. Technique of artificial reproduction of Botia superciliaris Günther[J]. Freshwater Fisheries, 2011,41(5):92-95.]
[10] 唐艳高, 徐大宝, 王运能, 等. 紫薄鳅的人工繁殖初步试验[J]. 中国水产, 2010, 1:41-43.
[11] 胡廷尖, 储张杰, 王雨辰, 等. 泥鳅规模化人工催产与孵化的研究[J]. 浙江海洋学院学报(自然科学版), 2012, 31(7):12-17.[HU T J, CHU Z J, WANG Y C, et al. Studies on the Artificial Large-Scale Propagation of Loach[J] Journal of Zhejiang Ocean University(Natural Science), 2012,31(7):12-17.]
[12] 朱光来, 赵子明. 泥鳅的人工繁殖与苗种培育初步研究[J]. 中国农学通报, 2014, 30(5):61-67.[ZHU G L, ZHAO Z M. The Preliminary Study on Artificial Propagation and Fry Rearing of Misgurnus anguillicaudatus[J]. Chinese Agricultural Science Bulletin,2014, 30(5):61-67.]
[13] 覃志彪,冯健,孙挺,等. 注射LHRHa对泥鳅血清性类固醇激素变化研究[J].水生生物学报, 2009, 33(6):1168-1172.[QIN Z B,FENG J,SUN T, et al. Hange Discipline of Sex Steroid Hormonesin Serum of Loach (Misgurnusanguillicaudatus) Infected Chorionic Gonadotropin Analogue (lhrha)[J]. acta hydrobiologica sinica, 2009, 33(6):1168-1172.]
[14] 王应天. 绒膜促性腺激素和畄族激素等对于蟾蜍卵巢的排卵与成熟的作用[J]. 实验生物学报, 1963, 8(3-4):517-537.[WANG Y T. Induced Ovulation and Maturation of the Oocytes with Human Chorionic Gonadotrophin and Steroid Hormones in the Toad, Bufo Bufo Gargarizans[J]. acta biologiae experimentalis sinica, 1963, 8(3-4):517-537.]
[15] 刘建康, 何碧悟. 中国淡水鱼类养殖学(第三版)[M]. 北京:科学出版社, 1992, 94-103.
[16] 伍献文, 钟麟. 鲩、青、鲢、鳙的人工繁殖在我国的进展和成就. 1964年北京科学讨论会的宣读.
[17] 刘筠. 家鱼人工繁殖研究工作的现状与展望(综评)[J]. 水产学报, 1965, 2(3):49-57.[LIU Y. On the Present Status Ani Future Prospect in the Artificial Propagation of Farm Fishes[J]. Journal of Fisheries of China, 1965, 2(3):49-57.]
[18] 危起伟, 李罗新, 杜浩,等. 中华鲟全人工繁殖技术研究[J]. 中国水产科学, 2013. 20(1):1-11.[WEI Q W, LI L X,DU H, et al. Research on technology for controlled propagation of cultured Chinese sturgeon (Acipenser sin-ensis)[J]. Journal of Fishery Sciences of China, 2013. 20(1):1-11.]
[19] QIN J G,MADON S P,CULVER D A. Effect of larval walleye (Stizostedion vitreum) and fertilization on the plankton community:implications for larval fish culture[J]. Aquaculture,1995,130:51-65.
[20] 韩茂森, 束蕴芳. 中国淡水生物图谱[M]. 北京:海洋出版社, 1995, 192-220.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed