Abstract: Exploring the temporal-spatial evolution of suburban rural tourism destinations and its determinants of spatial distribution is conducive to guiding orderly rural tourism development in suburban area，the hot area of rural tourism development; and it is helpful for guiding suburban rural tourism development practice and rural-revitalization-strategy policy making. 534 rural tourism destinations within Wuhan City’s rural urban fringe were analyzed and classified, and the mathematical statistics and spatial analysis methods including the nearest neighbor index, kernel density estimation and Hotspot analysis were fully applied to study the temporal-spatial distribution features and its influencing factors. The conclusions are as follows: (1) Rural tourism development in suburban Wuhan has experienced four distinct development periods; and the distribution of rural destination hot spots and cold spots in Wuhan has been changing dynamically; now, three tourism agglomeration areas have been formed in the northwest, west and south of Wuhan. (2) The types of rural tourism destinations in Wuhan are increasingly diversified, from the original two types (sightseeing tourism destinations and cultural tourism destinations) to current five types (sightseeing tourism destinations, farming tourism destinations, agricultural technology tourism destinations, cultural tourism destinations, and special village and town), and there are differences in the development speed, scale and agglomeration of different types of rural tourist destinations. Thereinto, sightseeing tourism destinations have the largest development scale, with a significant trend of concentrated development, forming a multi-core development distribution pattern; and farming tourism destinations started later, but with the fastest development speed and the highest degree of concentration；meanwhile, special villages and towns also experience some agglomeration distribution with indistinct agglomeration evolution trend, and the radiation range of the polar core are always small；cultural tourism destinations gradually evolved from scattered distribution to a spatial pattern combining "great dispersion" and "small agglomeration"；agricultural technology tourism destinations have been in the state of dispersion rather than agglomeration.(3) Distance from the central urban area, policies factor, traffic conditions, the hydrological factors and quality of rural tourism attractions, all are determinants of the spatial and temporal distribution characteristics of rural tourist destinations with different impacts on the spatial and temporal distribution of different types of tourist destinations. This research ended with some suburban rural tourism development references and suggestions.