Abstract: The spatial distribution of logistics enterprises determines the spatial pattern of urban logistics and affects the rational allocation of urban resources and production space.Based on the data of logistics enterprises in Shanghai since 1995, this study uses the measurement methods such as location quotient, average nearest neighbor, kernel density and colocation quotient to explore the location and evolution characteristics of various logistics enterprises. The results show that:(1) Due to the different resource endowments, location conditions and development orientation, the development level of logistics industry in Shanghai metropolitan area is obviously spacetime heterogeneity, and the difference of spacetime development level among various logistics enterprises in the region is significant. (2) The logistics enterprises in Shanghai metropolitan area are shifting from “central layout” to multicoreperiphery model. While logistics enterprises are concentrated and polarized in the central area, a large number of logistics enterprises are clustered and distributed outside the suburbs. The urban logistics space is expanding to the periphery.(3)Freight Forwarder and integrated logistics enterprises through the information sharing, zero inventory services and other factors, to promote mutual choice between enterprises; other types of logistics enterprises due to geographical division of labor, business locations scattered, there has been the location of mutual exclusion phenomenon.This research reveals the location characteristics of Shanghai metropolitan logistics space, which not only complements the theoretical research of urban logistics, but also provides reference for the development of urban logistics and logistics facilities in the future