Abstract: Under the background of green development, the way to make appropriate use of environmental regulation tools to deal with the relationship between tourism industry development and ecological environment protection is a hot issue in current research.By constructing the evaluation index system of regional environmental regulation and tourism ecological efficiency, using entropy weighting method and super efficiency SBM model, this paper calculates the value of environmental regulation and tourism ecological efficiency of urban agglomeration in the Yangtze River Delta from 2007 to 2017. On this basis, based on the natural breakpoint method, this paper analyzes the spatial pattern evolution of the two, then establishes a panel model, and uses the system generalized moment estimation to analyze the impact of environmental regulation on tourism ecological efficiency from the two dimensions of time and space.The conclusions are as follows:（1)The overall environmental governance of urban agglomerations has been strengthened, and the regional differences have gradually narrowed. From the perspective of spatial distribution, the spatial pattern changes greatly, which is reflected in the transfer and upgrading of high regulatory intensity areas from the central to the eastern and western marginal areas;（2)The overall level of tourism ecological efficiency of urban agglomerations is not high, showing a trend of first falling and then rising, and the spatial differences first expand and then narrow. Efficiency gradually forms the spatial distribution pattern of high-efficiency cluster cluster in the central of urban agglomeration;（3)There is a significant negative correlation between environmental regulation and tourism ecological efficiency in urban agglomeration, but with the increase of lag order, the negative impact of environmental regulation on green development of tourism industry is gradually weakening. From the spatial dimension, there are significant differences in the impact of urban environmental regulations on efficiency. Among them, the cities which are positively affected by environmental regulations are less and mainly distributed in the central area of urban agglomeration.