长江流域资源与环境 >> 2018, Vol. 27 >> Issue (09): 2031-2041.doi: 10.11870/cjlyzyyhj201809014

• 生态环境 • 上一篇    下一篇

铜绿微囊藻对有机毒物菲的生理生态响应研究

冯凡1,2,赵中华1*,陈晨2,田园2,郦倩玉1,龚雄虎1,3,叶晨昊2   

  1. ( 1.中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室,江苏 南京 210008;2.江苏科技大学环境与化学工程学院,江苏 镇江 212005; 3.湘潭大学资源与环境学院,湖南 湘潭 411105)
  • 出版日期:2018-09-20 发布日期:2018-11-09

PhysiEcological Responses of Microcystis aeruginosa to Phenanthrene Exposure

FENG Fan,ZHAO Zhonghua,CHEN Chen,TIAN Yuan,LI Qianyu,GONG Xionghu,YE Chenhao   

  1. (1. Nanjing Institute of Geography &Limnology Chinese Academy of Sciences, State Key Laboratory of Lake Science and Environment, Nanjing 210000, China;2. School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212005, China; 3.Xiangtan University College of Environment and Resources, Xiangtan 411105, China
  • Online:2018-09-20 Published:2018-11-09

摘要: 选取富营养化湖泊蓝藻水华常见优势种铜绿微囊藻(Microcystis aeruginosa,M. aeruginosa)为研究对象,采用室内暴露培养实验考察多环芳烃(Polycyclic aromatic hydrocarbons,PAHs)化合物菲(Phenanthrene,Phe)对铜绿微囊藻生长及生理特性的影响效应和作用机制。研究结果表明,低浓度Phe(0.05~0.2 mg/L)对铜绿微囊藻细胞生长有不同程度的促进作用,0.2 mg/LPhe促进作用最为显著(P<0.05);高浓度Phe(0.5~1.0 mg/L)显著抑制铜绿微囊藻生长(P<0.05)。铜绿微囊藻典型生理指标细胞光合作用率(Fv/Fm)、超氧化物歧化酶(SOD)、谷氨酰胺还原酶(GR)酶活性以及丙二醛(MDA)含量同步分析结果显示,低浓度Phe通过增强SOD、GR酶活性,降低铜绿微囊藻细胞内MDA含量,提高藻细胞光合作用率,进而促进细胞增殖;高浓度Phe则通过降低SOD、GR酶活性,增加藻细胞内MDA产量,减弱细胞光合作用,从而抑制细胞生长。对不同暴露时刻藻细胞的生长抑制率进行回归分析可得EC50(半抑制浓度)随着污染暴露时间增加而减低,1dayEC50为1.27 mg/L,12dayEC50降至0.65 mg/L,即高剂量Phe(0.5~1.0 mg/L)对微囊藻细胞生长的抑制作用随时间延长逐渐增强。结合低剂量浓度下Phe对铜〖JP+2〗绿微囊藻生长的促进作用分析,湖泊生态系统Phe污染长期暴露对铜绿微囊藻生长生理特性及优势种的形成和维持具有不可忽视的作用。其中,铜绿微囊藻细胞主要生理指标SOD与GR对Phe污染胁迫反应灵敏,可用作评价PAHs等有机毒物对浮游藻类生理生态影响的生物标记物

Abstract: The physiecological responses of Microcystis aeruginosa to different concentrations of phenanthrene (Phe) with different exposure time were studied. Results indicated that the growth rates of M. aeruginosa were stimulated under the lower doses of Phe with the range of 005-02 mg/L; compared to the control, the stimulation effects under 02 mg/L Phe was significant (P<005).Growth rates were significantly inhibited under the higher concentrations of Phe (05-10 mg/L) (P<005). Potential internal mechanisms were further investigated by analyzing several typical physiological parameters such as the photochemical efficiency (Fv/Fm), superoxide dismutase (SOD), glutathione reductase (GR) and malondialdehyde (MDA). Under the lower exposure concentrations (005-02 mg/L), the associated increase of the enzyme activities of SOD and GR, and the decrement of MDA contents would improve the photosynthesis and stimulate the growth rates.While for the higher exposure concentrations (05-10 mg/L), the growth rates were inhibited mainly by the decrement of SOD activities, GR activities and photosynthesis as well as the increase of MDA contents. The EC50 of Phe (1dayEC50 and 12dayEC50 were 127 mg/L and 065 mg/L, respectively) on the inhibition of the growth indicated the inhibition increased with the exposure time. Therefore, the development and maintenance of phytoplankton community dominated by M. aeruginosa, would more or less affected by the continuous inputs of Phe. Moreover, such physiological properties as SOD and GR could be used as efficient biomarkers of Phe pollution because of their high sensitivity

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 沈德福,史学正,吕成文,于东升. 江苏沿江地区土壤肥力空间分布及其区域对比研究[J]. 长江流域资源与环境, 2005, 14(3): 316 -321 .
[2] 宋玉芝,秦伯强, 高光. 附着生物对富营养化水体氮磷的去除效果[J]. 长江流域资源与环境, 2009, 18(2): 180 .
[3] 许峰, 祁士华, 高媛, 邢新丽. 绵阳市代表性点位土壤多环芳烃剖面分布特征[J]. 长江流域资源与环境, 2009, 18(2): 192 .
[4] 张榆霞,刘嘉麒,王立前. 漫湾电站建成后澜沧江下游水质变化[J]. 长江流域资源与环境, 2005, 14(4): 501 -506 .
[5] 陈 勇,陈国阶,杨定国. 岷江上游聚落分布规律及其生态特征——以四川理县为例[J]. 长江流域资源与环境, 2004, 13(1): 72 -77 .
[6] 解晓南,许朋柱,秦伯强. 太湖流域苏锡常地区地面沉降若干问题探析[J]. 长江流域资源与环境, 2005, 14(1): 125 -131 .
[7] 刘 健,陈 星,彭恩志,周学东. 气候变化对江苏省城市系统用电量变化趋势的影响[J]. 长江流域资源与环境, 2005, 14(5): 546 -550 .
[8] 彭长青,冯金飞,卞新民. 基于遗传算法和GIS的县域水田种植制度空间布局优化[J]. 长江流域资源与环境, 2006, 15(1): 66 -70 .
[9] 刘爱霞,刘正军,王 静. 基于PCA变换和神经元网络分类方法的中国森林制图研究[J]. 长江流域资源与环境, 2006, 15(1): 19 -24 .
[10] 郑明媚,李满春,毛 亮,黎韶光. GIS支持的县域人口迁移空间模型研究——以浙江省临安市为例[J]. 长江流域资源与环境, 2006, 15(3): 281 -286 .